The P1 region of the genome is encoding four viral structural proteins (VP4, VP2, VP3, and VP1). system to control FMDV and useful for vaccine development in the future. genus of the family. The genome is usually a compact positive-strand RNA about 8,300 nucleotides long with a single open reading frame (ORF) [6]. The genome is usually translated as a single ORF into a precursor polyprotein and the precursor protein is usually cleaved by viral coded proteases into both the intermediate and mature structural and nonstructural (NS) viral proteins. Based on the initial cleavage products, the genome ORF Bretylium tosylate is usually divided Bretylium tosylate into four regions including the L, P1, P2, and P3 region, respectively [7]. The P1 region of the genome is usually encoding four viral structural proteins (VP4, VP2, VP3, and VP1). Following the P1 region is the P2, encodes three viral NS proteins (2A, 2B, and 2C), and the P3 region, encodes NS proteins 3A, three copies of VPg (3B1, 3B2, and 3B3), 3C protease (3Cpro), and 3D polymerase (3Dpol). The protease 3C plays crucial role in the cleavage of viral structural proteins and enables Bretylium tosylate the proper folding and assembly of the FMDV capsid in the infected cells [7-9]. FMDV is one of the highly antigenic variable viruses, as a result of error-prone replication, and the lack of 3Dpol gene proofreading and postreplicative repair activities. Bretylium tosylate Therefore, the FMDV consists of the seven serotypes including type O, A, C, SAT-1, SAT-2, SAT-3, and Asia-1; 64 subtypes. Among the seven serotypes of FMDV, the serotype “O” is the most common and it is prevalent in China and its surrounding countries. Furthermore, Bretylium tosylate serotype O has been detected in South Korea, during the massive outbreaks of foot-and-mouth disease (FMD) in 2011 [10]. The development of FMDV BCLX vaccine is usually important to control the FMD outbreaks in many countries. Thus, a lot of different approaches have been attempted. At the beginning, the killed or inactivated vaccines have been used. However, FMDV vaccines like other killed antigens do not induce broadly reactive long-term protection; require multiple vaccinations to maintain good levels of herd immunity. Despite, conventional binary ethyleneimine inactivated vaccines emulsified with adjuvant have been widely used in Asia, Africa, and South America for effective control and eradication programs. Several novel approaches have been applied to develop option FMD vaccines, including construction of altered live-virus [11,12], biosynthetic proteins [13,14], synthetic peptides [15,16], naked DNA vectors [17,18], oral vaccine produced from transgenic plants [19,20] and recombinant viruses. Recombinant adenovirus [21-23], recombinant vaccinia computer virus [24], pseudorabies or fowlpox-vectored vaccine [25,26] and recombinant baculoviruses have been developed to express computer virus like particles (VLP) [27,28]. In the present study, we attempted to develop a novel strategy for FMDV vaccine using porcine reproductive and respiratory syndrome computer virus (PRRSV) replicon as a vector. Our results indicate that a PRRSV replicon vector expresses FMDV structural protein as well as N protein of PRRSV DH5 cells, and the sequences were analyzed using gene sequencing. FMDV gene made up of PRRSVK418DM and the N gene made up of plasmids were digested with transcription Replicon plasmids were isolated using a QIAfilter Plasmid Maxi Kit (Qiagen, Hilden, Germany) followed by identification by electrophoresis, restriction enzyme map identification. Ten micrograms of replicon plasmid was linearized by cleavage with the restriction enzyme either transcripts along with 10 g of total RNA isolated from MARC-145 cells by pulsing once using Bio-Rad Gene.